siRNA Delivery: From Lipids to Cell‐penetrating Peptides and Their Mimics
Identifieur interne : 001318 ( Main/Exploration ); précédent : 001317; suivant : 001319siRNA Delivery: From Lipids to Cell‐penetrating Peptides and Their Mimics
Auteurs : Matt Gooding [Royaume-Uni] ; Lorcan P. Browne [Royaume-Uni] ; Filipa M. Quinteiro [Royaume-Uni] ; David L. Selwood [Royaume-Uni]Source :
- Chemical Biology & Drug Design [ 1747-0277 ] ; 2012-12.
English descriptors
- Teeft :
- Acad, Acid particles, Amine, Amino acids, Amphipathic, Antisense, Arginine, Arginine residues, Assay, Biochem, Biochim biophys, Biol, Biophys, Cationic, Cationic lipid, Cationic liposomes, Cell membrane, Cellular uptake, Charge repulsion, Chem, Chem biol drug, Clinical trials, Covalently, Cpps, Dendrimers, Egfp, Electrostatic complexes, Endocytosis, Endosomal, Endosomal membrane, Endosome, Endosomes, Gapdh, Gooding, Hela, Hela cells, Heparin, Internalization, Intracellular, Kinase, Knockdown, Lipid, Lipophilic, Liposome, Lnps, Luciferase, Mammalian cells, Membrane, Mimic, Mitochondrial, Mrna, Mrna knockdown, Nanoparticles, Natl, Nucleic, Nucleic acids, Oligonucleotide, Oligonucleotides, Pathway, Penetratin, Pepfect6, Peptide, Polyarginine, Polymer, Primary cells, Proc, Proc natl acad, Receptor, Risc, Rnai, Sirna, Sirna delivery, Small molecules, Snalp, Snalps, Sulphate, Therapeutics, Toxicity, Transduction, Transfection, Translocation, Transporter, Tumour, Vivo, Vivo delivery, Wender.
Abstract
To deliver siRNA for therapeutic use, several hurdles must be addressed. Metabolic degradation must be blocked, and the RNAi cellular machinery is located in the cytoplasm, while double‐stranded siRNA is large, highly charged and impermeable to cell membranes. To date, the solutions to the delivery issues have mostly involved different forms of lipid particle encapsulation. Cell‐penetrating peptides and their mimics or analogues offer a different approach and this is an emerging field with the first in vivo examples now reported. Recent reports point to lipid receptors being involved in the cellular uptake of both types of transporter. This review examines the delivery of siRNA with a focus on cell‐penetrating peptides and their small molecule and oligomeric mimics. The current status of siRNA delivery methods in clinical trials is examined. It now seems that the goal of delivering siRNA therapeutically is achievable but will they form part of a sustainable healthcare portfolio for the future.
Url:
DOI: 10.1111/cbdd.12052
Affiliations:
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Metabolic degradation must be blocked, and the RNAi cellular machinery is located in the cytoplasm, while double‐stranded siRNA is large, highly charged and impermeable to cell membranes. To date, the solutions to the delivery issues have mostly involved different forms of lipid particle encapsulation. Cell‐penetrating peptides and their mimics or analogues offer a different approach and this is an emerging field with the first in vivo examples now reported. Recent reports point to lipid receptors being involved in the cellular uptake of both types of transporter. This review examines the delivery of siRNA with a focus on cell‐penetrating peptides and their small molecule and oligomeric mimics. The current status of siRNA delivery methods in clinical trials is examined. It now seems that the goal of delivering siRNA therapeutically is achievable but will they form part of a sustainable healthcare portfolio for the future.</div></front></TEI><affiliations><list><country><li>Royaume-Uni</li></country></list><tree><country name="Royaume-Uni"><noRegion><name sortKey="Gooding, Matt" sort="Gooding, Matt" uniqKey="Gooding M" first="Matt" last="Gooding">Matt Gooding</name></noRegion><name sortKey="Browne, Lorcan P" sort="Browne, Lorcan P" uniqKey="Browne L" first="Lorcan P." last="Browne">Lorcan P. Browne</name><name sortKey="Quinteiro, Filipa M" sort="Quinteiro, Filipa M" uniqKey="Quinteiro F" first="Filipa M." last="Quinteiro">Filipa M. Quinteiro</name><name sortKey="Selwood, David L" sort="Selwood, David L" uniqKey="Selwood D" first="David L." last="Selwood">David L. Selwood</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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